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    Invitrogen hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsdna. Cultured human neonatal dermal fibroblasts were fixed and permeabilized, with staining for tubulin (red fluorescence), actin (green fluorescence), and nuclei (blue-fluorescent you clicked on an external link, which has been disabled in order to keep your shopping session open. Note hoechst dye has poor solubility in water, so sonicate as necessary to dissolve. Dissolving hoechst dye in pbs is not recommended, but phosphate-containing buffers may be used with dilute solutions of the dye.


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    Unbound hoechst dye has a maximum emission in the 510540 nm range a green haze may be observed if too much dye is applied. . The 10 mgml hoechst stock solution may be stored at 26c for up to 6 months or at 20c for longer periods. Prepare the hoechst dye stock solution by dissolving the contents of one vial (100 mg) in 10 ml of deionized water (dih o) to create a 10 mgml (16. Dissolving hoechst dye in pbs is not recommended, but phosphate-containing buffers may be used with dilute solutions of the dye. Invitrogen hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsdna. Cultured human neonatal dermal fibroblasts were fixed and permeabilized, with staining for tubulin (red fluorescence), actin (green fluorescence), and nuclei (blue-fluorescent you clicked on an external link, which has been disabled in order to keep your shopping session open. Note hoechst dye has poor solubility in water, so sonicate as necessary to dissolve. Prepare the hoechst staining solution by diluting the hoechst stock solution 12,000 in pbs. ).

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